《植物生理学报》 2017, 53(2): 234-240

家独行菜的组织培养与高频再生

赵杨阳, 刘佳钰, 马征, 姜雪, 黄萱^*

西部资源与现代生物技术省部共建教育部重点实验室, 陕西省生物技术重点实验室, 西北大学生命科学学院, 西安710069

通信作者：黄；E-mail: xuanhuang@nwu.edu.cn

摘　要：

以家独行菜萌发苗的茎和叶作为外植体, 利用含有多种不同植物生长调节物质及浓度配比的MS培养基分别诱导产生愈伤组织、丛生芽和再生根, 建立了组织培养和高频离体再生体系。结果表明: 最适外植体为苗龄14 d的家独行菜萌发苗茎段, 其诱导愈伤组织的最适培养基为MS+1.5 mg·L^-1 2,4-D+0.5 mg·L^-1 6-BA+0.1 mg·L^-1 NAA, 诱导率可达95.4%; 诱导不定芽的最适培养基为MS+0.5 mg·L^-1 2,4-D+1.5 mg·L^-1 6-BA+0.2 mg·L^-1 NAA+0.1 mg·L^-1 TDZ, 诱导率可达89.9%; 在MS+0.6 mg·L^-1 NAA的培养基中可获得最佳生根率, 大约有87.5%的再生苗生长出健康的根系。炼苗后将长势良好的再生苗进行移栽, 存活率可达90%。

关键词：家独行菜; 组织培养; 高频再生; 植物生长调节剂

收稿：2016-12-29   修定：2017-01-23

资助：国家自然科学基金(31300223)、国家自然科学基金基地建设能力提高项目(J1210063)、陕西省自然科学基础计划(2016JM3001)、高等学校博士学科点专项科研基金(20126101120019)、陕西省教育厅重点科研计划(15JS110)、省级大学生创新创业训练计划(1030)和教育部留学回国人员科研启动基金资助项目(教外司留[2013]693号)。

Tissue culture and high-frequency regeneration of Lepidium sativum

ZHAO Yang-Yang, LIU Jia-Yu, MA Zheng, JIANG Xue, HUANG Xuan^*

Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, Shaanxi Provincial Key Laboratory of Biotechnology, College of Life Sciences, Northwest University, Xi’an 710069, China

Corresponding author: HUANG Xuan; E-mail: xuanhuang@nwu.edu.cn

Abstract:

The stems and leaves of Lepidium sativum seedlings were used as explants, MS medium compounding proportions of different plant growth regulators were studied on callus induction, adventitious bud differentiation, and root regeneration, thereafter an efficient plant-regeneration system was established by in vitro L. sativum culture. On MS medium containing 1.5 mg·L^-1 2,4-D, 0.5 mg·L^-1 6-BA and 0.1 mg·L^-1 NAA, stems could be induced to product calli, and callus induction ratio reached to 95.4%. On MS medium containing 0.5 mg·L^-1 2,4-D, 1.5 mg·L^-1 6-BA, 0.2 mg·L^-1 NAA and 0.1 mg·L^-1 TDZ, adventitious buds could be regenerated from calli, and the differentiation ratio was 89.9%. Well-grown adventitious buds were inoculated on MS medium containing 0.6 mg·L^-1 NAA, and 87.5% rooting frequency was obtained. Regenerated plantlets grew well after being transplanted to soil. Moreover, the survival ratio of plantlets reached 90%.

Key words: Lepidium sativum; tissue culture; high-frequency regeneration; plant growth regulators